Кемерово, Кемеровская область, Россия
Biosensors are promising analytical tools applicable in clinical diagnostics, food industry, environmental monitoring, and other areas in which rapid and reliable analyses are needed. This review covers the basic types of biosensors and their designs and general operating principles. A classification of biosensors according to the type of transducer they involve and according to the nature of the biological entity used as the recognition element is presented. Methods of immobilization of biological components, namely, adsorption, microencapsulation, inclusion, cross-linking, and covalent binding are briefly characterized. The main areas of application of biosensors in the food industry—food safety and quality assessment, process monitoring, and others—are considered.
biosensor, transducer, immobilization, foods
INTRODUCTION
The issue of food safety and quality is becoming increasingly challenging. This is due to the fact that hazardous foods that can contain toxic substances, including those of anthropogenic origin, appear on the market. Environmental pollution caused by sewage from industrial and agricultural enterprises, which contains residual pesticides, fertilizers, growth stimulants, and other harmful substances, leads to accumulation of toxicants in fish, meat, and dairy foods. In addition, in the 1980s some countries began extensive studies in the design of genetically modified organisms (GMOs) intended for the production of foods, animal feeds, and pharmaceutical and veterinary preparations. This highlights the need for extensive supervision aimed at revealing the strains that were created in violation of safety principles and at checking whether a product obtained using a GMO is identical to its natural prototype.
In view of the aforesaid, increasing attention is being focused on development of sufficiently sensitive and selective express analysis methods. Prominent examples of analytical systems combining all of the above merits are biosensors [1–4].
GENERAL OPERATING PRINCIPLES AND DESIGN OF BIOSENSORS
Biosensors are analytical devices that employ sensitive biological materials to "recognize" certain molecules and provide information on their presence and amount as a signal convenient for recording and processing [4–10].
Any biosensor consists of the following three basic components: recognition element, which is a biose-lective membrane involving various biological structures; physical transducer; electronic system for signal amplification and recording and for user-friendly data representation [4, 7–17]. Figure 1 presents a general scheme of a biosensor.
1. Babkina, S.S., Ulakhovich, N.A., Zyavkina, Yu.I., Moiseeva, I.N., Filyushina, E.E., Nikitin, E.V., and Zagumennov, V.A., Khimiya i khimicheskaya tekhnologiya (Chemistry and chemical engineering), 2005, vol. 48, no. 1, pp. 22-25.
2. Liu, J., Brown, A.K., Meng, X., Cropek, D.M., Istok, J.D., Watson, D.B., Lu, Y., and Liu, J., Proceedings of the National Academy of Sciences of the United States of America, 2007, vol. 104, no. 7, pp. 2056-2061.
3. Evtyugin, G.A., Budnikov, G.K., and Porfir´eva, A.V., Rossiiskii khimicheskii zhurnal (Russian chemical journal), 2008, vol. 52, no. 2, pp. 66-79.
4. Karyakin, A.A., Ulasova, E.A., Vagin, M.Yu., and Karyakina, E.E., Sensor (Sensor), 2002, no. 1, pp. 16-24.
5. Korotkaya, E.V., Biosensory na osnove kollodievykh plenok: Monografiya (Biosensors Based on Collodion Films: A Monograph), Kemerovo: Kemerov. Tekhnol. Inst. Pishchevoi Prom-sti., 2011.
6. Evtyugin, G.A., Budnikov, G.K., and Nikol´skaya, E.B., Russian Chemical Reviews, 1999, vol. 68, no. 12, pp. 1041-1064.
7. Budnikov, G.K., Sorosovskii obrazovatel´nyi zhurnal (Soros educational journal), 1996, no. 12, pp. 26-32.
8. Eggibs, B., Chemical Sensors and Biosensors, New York: Wiley, 2002.
9. Biosensors: Fundamentals and Applications, Turner, A., Karube, I., and Wilson, G., Eds., Oxford: Oxford Univ. Press, 1987.
10. Rastislav Monošíka, Miroslav Streďanskýb, and Ernest Šturdík, Acta Chimica Slovaca, 2012, vol. 5, no. 1, pp. 109-120. doihttps://doi.org/10.2478/v10188-012-0017-z
11. Cavalcanti, A., Shirinzadeh, B., Zhang, M., and Kretly, L.C., Sensors, 2008, vol. 8, pp. 2932-2958.
12. Lojou, É. and Bianko, P., Journal of Electroceramics, 2006, vol. 16, no..1, pp. 79-91.
13. Kitova, A.E., Extended Abstract of Cand. Sci. (Biol.) Dissertation, Saratov: Institute of Biochemistry and Physiology of Plants and Microorganisms, 2009.
14. Chunhui Dai and Seokheun Choi, Open Journal of Applied Biosensor, 2013, vol. 2, pp. 83-93.
15. Mohanty, S.P. and Kougianos, E., IEEE Potentials, 2006, vol. 25, no. 2, pp. 35-40. doihttps://doi.org/10.1109/MP.2006.1649009
16. Lei, Y., Chen, W., and Mulchandani, A., Analytica Chimica Acta, 2006, vol. 568, nos. 1-2, pp. 200-210. doihttps://doi.org/10.1016/j.aca.2005.11.065
17. D’Souza, S.F., Biosensor and Bioelectronics, 2001, vol. 16, no. 6, pp. 337-353. doihttps://doi.org/10.1016/S0956-5663(01)00125-7.
18. Lud, S.Q., Nikolaides, M.G., Haase, I., Fischer, M., and Bausch, A.R., ChemPhysChem, 2006, vol. 7, no. 2, pp. 379-384.
19. Ha Minh Hiep, Tatsuro Endo, Kagan Kerman, Miyuki Chikae, Do-Kyun Kim, Shohei Yamamura, Yuzuru Takamura, and Eiichi Tamiya, Science and Technology of Advanced Materials, 2007, vol. 8, pp. 331-338.
20. Kitova, A.E. Alferov, V.A., and Ponamoreva, O.N., in Mikrobnye biokatalizatory i perspektivy razvitiya fermentnykh tekhnologii v pererabatyvayushchikh otraslyakh APK (Microbial Biocatalysts and Outlooks for the Development of Enzyme Technologies in Processing Sectors of Agribusiness), Moscow: Pishchepromizdat, 2004, pp. 255-262.
21. Reshetilov, A.N., Kitova, A.E. Alferov, V.A., and Ponamoreva, O.N., Biotekhnologiya-2003: Tezisy dokladov seminara-prezentatsii innovatsionnykh nauchno-tekhnicheskikh proektov (Biotechnology 2003: Workshop and Presentation of Innovative Research and Development Projects), Pushchino, Moscow oblast, 2003, pp. 64-65.
22. Shevtsova, O.A., Zbirnik naukovikh prats´ Kharkivs´kogo universitetu povitryanikh sil (Transactions of the Kharkiv university of air forces), 2010, issue 1 (23), pp. 201-205.
23. Spiridonova, V.A., Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, 2010, vol. 4, no. 2, pp. 138-149.
24. Juan Zhang, Lihua Wang, Dun Pan, Shiping Song, Freddy Y.C. Boey, Hua Zhang, and Chunhai Fan, Small, 2008, vol. 4, no. 8, pp. 1196-1200.
25. Lui, C., Cady, N.C., and Batt, C.A., Sensors, 2009, vol. 9, pp. 3713-3744.
26. Reshetilov, A.N., Ponamoreva, O.N., Arlyapov, V.A., Alferov, V.A., Rogova, G.V., Blokhin, I.V., and Chepkova, I.F., Voda: khimiya i ekologiya (Water: chemistry and ecology), 2008, no. 3, pp. 23-30.
27. Perov, S.N., Kalach, A.V., and Meshcheryakova, O.L., Sorbtsionnye i khromatograficheskie protsessy (Sorption and chromatographic processes), 2008, vol. 8, no. 6, pp. 1008-1012.
28. Reshetilov, A.N., Applied Biochemistry and Microbiology, 2005, vol. 41, no. 5, pp. 442-449.
29. Deryabin, D.G. and Aleshina, E.S., Applied Biochemistry and Microbiology, 2008, vol. 44, no. 3, pp. 292-296.
30. Deryabin, D.G. and Aleshina, E.S., Gigiena i sanitariya (Hygiene and sanitation), 2008, no. 4, pp. 83-86.
31. Lavrinenko, I.A. and Lavrinenko, V.A., Vestnik Novosibirskogo gosudarstvennogo universiteta, seriya: Biologiya, klinicheskaya meditsina (Bulletin of Novosibirsk State University, series: Biology, clinical medicine), 2007, vol. 5, no. 1, pp. 118-126.
32. Zubov, V.P., Ivanov, A.E., Zhigis, L.S., Rapoport, E.M., Markvicheva, E.A., Lukin, Yu.V., and Zaitsev, S.Yu., Bioorganicheskaya khimiya (Bioorganic chemistry), 1999, vol. 25, no. 11, pp. 868-880.
33. Koval´chuk, M.V., Klechkovskaya, V.V., and Feigin, L.A., Priroda (Nature), 2003, no. 12, pp. 1-12.
34. Madou, M., Fundamentals of Microfabrication: The Science of Miniaturization, Boca Raton, Fla.: CRC, 2002, pp. 62-63.
35. Kaifer, A. and Gómez-Kaifer, M., Supramolecular Electrochemistry, Weinheim: Wiley-VCH, 1999, pp. 191-193.
36. Schramm, L., Dictionary of Nanotechnology, Colloid and Interface Science, Weinheim: Wiley-VCH, 2008.
37. Gol´dfarb, O.E., Extended Abstract of Cand. Sci. (Chem.) Dissertation, Kazan: Kazan State University, 2005.
38. Ermolaeva, T.N., Kalmykova, E.N., and Shashkanova, O.Yu., Rossiiskii khimicheskii zhurnal (Russian chemical journal), 2008, vol. 52, no. 2, pp. 17-29.
39. Analytical Chemistry: A Modern Approach to Analytical Science, Kellner, R., Mermet, J.-M., Otto, M., Valcarcel, M., and Widmer, H., Eds., Weinheim: Wiley-VCH, 2004.
40. Shlyapnikov, Yu.M., Extended Abstract of Cand. Sci (Chem.) Dissertation, Moscow: Moscow State University, 2010.
42. Vaefolomeev, S.D., Sorosovskii obrazovatel´nyi zhurnal (Soros educational journal), 1997, no. 1, pp. 45-49.
43. Luong, J.H.T., Groom, C.A., and Male, K.B., Biosensors and Bioelectronics, 1991, vol. 6, pp. 547-554.
44. Kopa, Yu. and Momynaliev, K., Nanoindustriya (Nanoindustry), 2007, no. 1, pp. 28-33.
45. Rumayor V.G, Iglesias, E.G, Galán, O.R, and Cabezas, L.G., Aplicaciones de biosensores en la industria agroalimentaria, Madrid: Comunidad de Madrid y la Universidad Complutense de Madrid, 2005.
46. Valimaa, A., Kivisto, A.T., Leskinen, P.I., and Karp, M.T., Journal of Microbiological Methods, 2010, vol. 80, no. 1, pp. 44-48.
47. Kalmykova, E.N., Melikhova, E.V., Dergunova, E.S., Eremin, S.A., and Ermolaeva, T.N., Sorbtsionnye i khromatograficheskie protsessy (Sorption and chromatographic processes), 2004, vol. 4, no. 5, pp. 597-605.
48. Pohanka, M., Pavlis, O., and Skladal P., Sensors, 2007, vol. 7, pp. 341-353.
49. Kalmykova, E.N. and Ermolaeva, T.N., Izvestiya vysshikh uchebnykh zavedenii, seriya: Khimiya i khimicheskaya tekhnologiya (Bulletin of higher education institutions, series: Chemistry and chemical engineering), 2005, vol. 48, no. 12, pp. 76-80.
50. Ermolaeva, T.N. and Kalmykova, E.N., P´ezokvartsevye sensory: Analiticheskie vozmozhnosti i perspektivy (Piezoelectric Quartz Crystal Sensors: Analytical Potential and Prospects), Lipetsk: Lipetsk. Gos. Tekh. Univ., 2007.
51. Lebedev, M.Yu., Eremin, S.A., Skladal, P., Analytical Letters, 2004, vol. 36, pp. 2443-2457.
52. Nartova, Yu.V., Eremin, S.A., and Ermolaeva, T.N., Sorbtsionnye i khromatograficheskie protsessy (Sorption and chromatographic processes), 2006, vol. 6, no. 5, pp. 764-772.
53. Nartova, Yu.V., Eremin, S.A., and Ermolaeva, T.N., Rossiiskaya shkola-konferentsiya molodykh uchenykh "Ekotoksikologiya: Sovremennye bioanaliticheskie sistemy, metody i tekhnologii" ("Ecotoxicology: Advanced Bionalytical Systems, Methods, and Technologies," Russian Young Scientists´ School and Conference), Pushchino, Moscow oblast, 2006, pp. 68-70.
54. Nartova, Yu.V., Mezhdunarodnaya konferentsiya molodykh uchenykh po fundamental´nym naukam "Lomonosov-2007", Khimiya ("Lomonosov 2007," International Young Scientists´ Conference on Fundamental Sciences, Section: Chemistry), Moscow, 2007, vol. 1, p. 39.
55. Dergunova, E.S. and Ermolaeva, T.N., Vserossiiskaya studencheskaya nauchno-tekhnicheskaya shkola-konferentsiaya "Imzhenernye nauki - zashchite okruzhayushchei sredy" ("Engineering Science for Environmental Protection," Russian National Students´ Science & Technology School and Conference), Tula, 2006, pp. 136-140.
56. Ermolaeva, T.N., Dergunova, E.S., Kalmykova, E.N., and Eremin, S.A., Journal of Analytical Chemistry, 2006, vol. 61, no. 6, pp. 609-613.
57. Kalmykova, E.N., Melikhova, E.V., Eremin, S.A., and Ermolaeva, T.N., Antibiotiki i khimioterapiya (Antibiotics and chemotherapy), 2004, vol. 49, no. 1, pp. 8-13.
58. Melikhova, E.V., Kalmykova, E.N., Eremin, S.A., and Ermolaeva, T.N., Journal of Analytical Chemistry, 2006, vol. 61, no. 7, pp. 687-693.
59. Yüce, M., Nazir, H., and Dönmez, G., Biosensors and Bioelectronics, 2010, vol. 26, no. 2, pp. 321-326.
60. Yüce, M., Nazir, H., and Dönmez, G., Bioresource Technology, 2010, vol. 101, no. 19, pp. 7551-7555.
61. Yüce, M., Nazir, H., and Dönmez, G., New Biotechnology, 2011, vol. 28, no. 4, pp. 356-361.
62. Alpat, S., Aplat, S.K., Cadirci, B.H., Yasa, I., and Telefoncu, A., Sensor and Actuators B: Chemical, 2008, vol. 134, no. 1, pp. 175-181.
63. Yüce, M., Nazir, H., and Dönmez, G., Bioelectrochemistry, 2010, vol. 79, no. 1, pp. 66-70.
64. Ravikumar, S., Ganesh, I., Yoo, I., and Hong, S., Process Biochemistry, 2012, vol. 47, no. 5, pp. 758-765.
65. Liu, P., Huang, Q., and Chen, W., Environmental Pollution, 2012, vol. 164, pp. 66-72.
66. Barrocas, P.R.G., Landing, W.M., and Hudson, R.J.M., Journal of Environmental Sciences, 2012, vol. 22, no. 8, pp. 1137-1143.
67. Singh, J. and Mittal, S.K., Sensors and Actuators B: Chemical, 2012, vol. 165, no. 1, pp. 48-52.
68. Reshetilov, A.N. and Kitova, A.E. http://cbio.ru/page/43/id/4791/.
69. Park, Je-K., Yee, H.-J., Lee, K. S., Lee, W.-Y., Shin, M.-C., Kim, T.-H., and Kim, S.-R., Analytica Chimica Acta, 1999, vol. 390, pp. 83-91.
70. Morozova, N.O., Ashin, V.V., Trotsenko, Yu.A., and Reshetilov, A.N., Applied Biochemistry and Microbiology, 1999, vol. 35, no. 5, pp. 537-541.
71. Akyilmaz, E. and Dinckaya, E., Biosensors and Bioelectronics, 2005, vol. 20, no. 7, pp. 1263-1269.
72. Valach, M., Katrlik, J., Sturdik, E., and Gemeiner, P., Sensors and Actuators B: Chemical, 2009, vol. 138, no. 2, pp. 581-586.
73. Hammerle, M., Hilgert, K., Marcus, A.H., and Ralf, M., Sensors and Actuators B: Chemical, 2011, vol. 158, no. 1, pp. 313-318.
74. Wen, G.M., Shuang, S.M., Dong, C., and Choi, M.F., Chinese Chemical Letters, 2012, vol. 23, no. 4, pp. 481-483.
75. Reshetilov, A.N., Kitova, A.E., Alferov, V.A., Ponamoreva, O.N., Kuzmichev, A. V., and Ezhkov, A.A., Biotekhnologiya-2003: Tezisy dokladov seminara-prezentatsii innovatsionnykh nauchno-tekhnicheskikh proektov (Biotechnology 2003: Workshop and Presentation of Innovative Research and Development Projects), Pushchino, Moscow oblast, 2003, pp. 64-65.
76. Li, L., Liang, B., Li, F., Shi, J., Mascini, M., Lang, Q., and Liu, A., Biosensors and Bioelectronics, 2013, vol. 42, no. 12, pp. 156-162.
77. Alferov, V.A., Arlyapov, V.A., and Kamanin, S.S., Izvestiya Tul´skogo gosudarstvennogo universiteta, seriya: Estestvennye nauki (Bulletin of Tula State University, series: Natural sciences), 2010, no. 2, pp. 247-255.
78. Il´nitskii, M.Yu. and Zaitsev, M.G., III Obshcherossiiskaya studenscheskaya elektronnaya nauchnaya konferentsiya "Studenscheskii nauchnyi forum" ("Students´ Scientific Forum," III Russian National Students´ e-Conference), 2011. http://www.rae.ru/forum2011/133/
79. Zaitsev, M.G., Extended Abstract of Cand. Sci. (Chem.) Dissertation, Moscow: Moscow State University of Fine Chemical Technologies, 2013.
80. Khitrova, Yu.S. and Indzhigiya, E.Yu., III Obshcherossiiskaya studenscheskaya elektronnaya nauchnaya konferentsiya "Studenscheskii nauchnyi forum" ("Students´ Scientific Forum," III Russian National Students´ e-Conference), 2011. http://www.rae.ru/forum2011/133/
81. Sibirtsev, I.A., Kamanin, S.S., and Arlyapov, V.A., III Obshcherossiiskaya studenscheskaya elektronnaya nauchnaya konferentsiya "Studenscheskii nauchnyi forum" ("Students´ Scientific Forum," III Russian National Students´ e-Conference), 2011. http://www.rae.ru/forum2011/133/
82. Panagopoulou Maria, A., Stergiou Dimitrios, V., and Roussis Ioannis, G., Analytical Chemistry, 2010, vol. 82, pp. 8629- 8636
83. Korneyeva, L.Kh., Borisova, A.V., Yashina, E.I., Karyakina, E.E., Voronin, O.G., Kosnier, S.. and Karyakin, A.A., Moscow University Chemistry Bulletin, 2010, vol. 51, no. 1, pp. 49-56.
84. Kolesnikov, P.S., Extended Abstract of Cand. Sci. (Vet.) Dissertation, Moscow: Russian Academy of Agricultural Sciences, 2005.
85. Babu, V.R.S., Patra, S., Karanth, N.G., Kumar, M.A., and Thakur, M.S., Analytica Chimica Acta, 2007, vol. 582, no. 2, pp. 329-334.
86. Minunni, M., Tombelli, S., Mariotti, E., and Mascini, M., Fresenius Journal of Analytical Chemistry, 2001, vol. 369, no. 7/8, pp. 589-593.
87. Minunni, M., Tombelli, S., Pratesi, S., and Mascini, M., Analytical Letters, 2001, vol. 34, no. 6, pp. 825-840.
88. Korbut, O., Bučková, M., Labuda, J., and Gründler, P., Sensors, 2003, vol. 3, pp. 1-10.
89. Labuda, J., Buckova, M., Heilerova, L., Silhar, S., and Stepanek, I., Analytical and Bioanalytical Chemistry, 2003, vol. 376, pp. 168-173.
