Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity

Haimoud S. Ali; Rachida Allem

doi:10.21603/2308-4057-2023-1-549

UDC

ALGERIAN DATE PALM (PHOENIX DACTYLIFERA L.) FRUIT CULTIVARS: HPLC FINGERPRINTING AND ANTIBACTERIAL ACTIVITY Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity

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Published in Foods and Raw Materials · Pages 17–24 · Rubric: Research Article

DOI: https://doi.org/10.21603/2308-4057-2023-1-549

Received: 01.01.2022 Accepted: 05.04.2022 Published: 17.03.2023 Language of publication: ENG

Authors

Haimoud S. Ali

ORCID

Rachida Allem

ORCID

Haimoud S. Ali

Hassiba Benbouali University of Chlef Algeria · Chlef

ORCID

Rachida Allem

Hassiba Benbouali University of Chlef Algeria · Chlef

ORCID

ABSTRACT

The abusive use of antibiotics causes the destruction of intestinal flora and the proliferation of antibiotic-resistant pathogens. Date palm is used in traditional medicine in the Saharan regions due to its biological properties. The study aimed to identify the phytochemical composition and assess the antibacterial activity of the methanolic extracts of three date cultivars from Algeria. Their total phenolic, flavonoid, and flavonol contents were measured spectrophotometrically. The phytochemical screening was conducted by HPLC fingerprinting using twenty-three pure phenolic compounds as standards. The antibacterial activity against pathogenic bacterial species was assessed using the disk diffusion method. The colorimetric methods showed that the total phenolic, flavonoid, and flavonol contents ranged from 2.13 ± 0.09 to 2.67 ± 0.02 mg GAE/100 g DW, 1.33 ± 0.21 to 1.55 ± 0.13 mg CEQ/100 g DW, and 0.41 ± 0.23 to 0.47 ± 0.05 mg REQ/100 g DW, respectively. HPLC fingerprinting showed that the extracts of date cultivars served as an excellent source of bioactive compounds (gallic acid, tannic acid, ferulic acid, vanillin, caffeine, quercetin, luteolin, rutin, aspegenin, isorhamnetin, and hesperidin). They also exhibited an antibacterial potential with an inhibition zone diameter ranging from 8.40 to 12.50 mm. The results clearly demonstrate the antibacterial potency of date palm fruits, which could be attributed to their considerable content of phenolic compounds such as gallic acid, rutin, quercetin, and luteolin.

KEYWORDS

Phoenix dactylifera L., high-performance liquid chromatography fingerprinting, phenolic compounds, flavonoids, flavonol, secondary metabolites

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1. Benkerrou F, Bachir M, Amrane M, Louaileche H. Ultrasonic-assisted extraction of total phenolic contents from Phoenix dactylifera and evaluation of antioxidant activity: statistical optimization of extraction process parameters. Journal of Food Measurement and Characterization. 2018;12(3):1910-1916. https://doi.org/10.1007/s11694-018-9805-5

2. Echegaray N, Pateiro M, Gullón B, Amarowicz R, Misihairabgwi JM, Lorenzo JM. Phoenix dactylifera products in human health - A review. Trends in Food Science and Technology. 2020;105:238-250. https://doi.org/10.1016/j.tifs.2020.09.017

3. Younas A, Naqvi SA, Khan MR, Shabbir MA, Jatoi MA, Anwar F, et al. Functional food and nutra-pharmaceutical perspectives of date (Phoenix dactylifera L.) fruit. Journal of Food Biochemistry. 2020;44(9). https://doi.org/10.1111/jfbc.13332

4. Al-Asmari AK, Al-Said MS, Abbasmanthiri R, Al-Buraidi A, Ibrahim KE, Rafatullah S. Impact of date palm pollen (Phoenix dactylifera) treatment on paracetamol-induced hepatorenal toxicity in rats. Clinical Phytoscience. 2020;6. https://doi.org/10.1186/s40816-020-0151-x

5. El Abed H, Chakroun M, Fendri I, Makni M, Bouaziz M, Drira N, et al. Extraction optimization and in vitro and in vivo anti-postprandial hyperglycemia effects of inhibitor from Phoenix dactylifera L. parthenocarpic fruit. Biomedicine and Pharmacotherapy.2017;88:835-843. https://doi.org/10.1016/j.biopha.2017.01.129

6. Kchaou W, Abbes F, Mansour RB, Blecker C, Attia H, Besbes S. Phenolic profile, antibacterial and cytotoxic properties of second grade date extract from Tunisian cultivars (Phoenix dactylifera L.). Food Chemistry. 2016;194:1048-1055. https://doi.org/10.1016/j.foodchem.2015.08.120

7. Mirza MB, Elkady AI, Al-Attar AM, Syed FQ, Mohammed FA, Hakeem KR. Induction of apoptosis and cell cycle arrest by ethyl acetate fraction of Phoenix dactylifera L. (Ajwa dates) in prostate cancer cells. Journal of Ethnopharmacology. 2018;218:35-44. https://doi.org/10.1016/j.jep.2018.02.030

8. Hussain MI, Farooq M, Syed QA. Nutritional and biological characteristics of the date palm fruit (Phoenix dactylifera L.) - A review. Food Bioscience. 2020;34. https://doi.org/10.1016/j.fbio.2019.100509

9. Siddiqi SA, Rahman S, Khan MM, Rafiq S, Inayat A, Khurram MS, et al. Potential of dates (Phoenix dactylifera L.) as natural antioxidant source and functional food for healthy diet. Science of the Total Environment. 2020;748. https://doi.org/10.1016/j.scitotenv.2020.141234

10. Qadir A, Shakeel F, Ali A, Faiyazuddin M. Phytotherapeutic potential and pharmaceutical impact of Phoenix dactylifera (date palm): Current research and future prospects. Journal of Food Science and Technology. 2019;57(4):1191-1204. https://doi.org/10.1007/s13197-019-04096-8

11. Biglari F, AlKarkhi AFM, Easa AM. Antioxidant activity and phenolic content of various date palm (Phoenix dactylifera) fruits from Iran. Food Chemistry. 2008;107(4):1636-1641. https://doi.org/10.1016/j.foodchem.2007.10.033

12. Al-Farsi M, Alasalvar C, Morris A, Baron M, Shahidi F. Comparison of antioxidant activity, anthocyanins, carotenoids, and phenolics of three native fresh and sun dried date (Phoenix dactylifera L.) varieties grown in Oman. Journal of Agricultural and Food Chemistry. 2005;53(19):7592−7599. https://doi.org/10.1021/jf050579q

13. Benmeddour Z, Mehinagic E, Meurlay DL, Louaileche H. Phenolic composition and antioxidant capacities of ten Algerian date (Phoenix dactylifera L.) cultivars: A comparative study. Journal of Functional Food. 2013;5(1):346-354. https://doi.org/10.1016/j.jff.2012.11.005

14. Perveen K, Bokhari NA, Soliman DAW. Antibacterial activity of Phoenix dactylifera L. leaf and pit extracts against selected Gram negative and Gram positive pathogenic bacteria. Journal of Medicinal Plants Research. 2012;6(2):296-300.

15. Alam M, Alhebsi MSR, Ghnimi S, Kamal-Eldin A. Inability of total antioxidant activity assays to accurately assess the phenolic compounds of date palm fruit (Phoenix dactylifera L.). NFS Journal. 2021;22:32-40. https://doi.org/10.1016/j.nfs.2021.01.001

16. Kadum H, Abdul Hamid A, Abas F, Ramli NS, Sabo Mohammed K, Muhialdin BJ, et al. Bioactive compounds responsible for antioxidant activity of different varieties of date (Phoenix dactylifera L.) elucidated by 1H-NMR based metabolomics. International Journal of Food Properties. 2019;22(1):462-476. https://doi.org/10.1080/10942912.2019.1590396

17. Ali Haimoud S, Allem R, Merouane A. Antioxidant and anti-inflammatory properties of widely consumed date palm (Phoenix dactylifera L.) fruit varieties in Algerian oases. Journal of Food Biochemistry. 2016;40(4):463-471. https://doi.org/10.1111/jfbc.12227

18. Sadeq O, Mechchate H, Es-Safi I, Bouhrim M, Jawhari FZ, Ouassou H, et al. Phytochemical screening, antioxidant and antibacterial activities of pollen extracts from Micromeria fruticosa, Achillea fragrantissima, and Phoenix dactylifera. Plants. 2021;10(4). https://doi.org/10.3390/plants10040676

19. Souli I, Jemni M, Rodríguez-Verástegui LL, Chaira N, Artés F, Ferchichi A. Phenolic composition profiling of Tunisian 10 varieties of common dates (Phoenix dactylifera L.) at tamar stage using LC-ESI-MS and antioxidant activity. Journal of Food Biochemistry. 2018;42(6). https://doi.org/10.1111/jfbc.12634

20. Alshwyeh HA. Phenolic profiling and antibacterial potential of Saudi Arabian native date palm (Phoenix dactylifera) cultivars. International Journal of Food Properties. 2020;23(1):627-638. https://doi.org/10.1080/10942912.2020.1751196

21. Kchaou W, Abbès F, Mansour RB, Blecker C, Attia H, Besbes S. Phenolic profile, antibacterial and cytotoxic properties of second grade date extract from Tunisian cultivars (Phoenix dactylifera L.). Food Chemistry. 2016;194:1048-1055. https://doi.org/10.1016/j.foodchem.2015.08.120

22. Al Harthi SS, Mavazhe A, Al Mahroqi H, Khan SA. Quantification of phenolic compounds, evaluation of physicochemical properties and antioxidant activity of four date (Phoenix dactylifera L.) varieties of Oman. Journal of Taibah University Medical Sciences. 2015;10(3):346-352. https://doi.org/10.1016/j.jtumed.2014.12.006

23. El Sohaimy SA, Abdelwahab AE, Brennan CS, Aboul-enein AM. Phenolic content, antioxidant and antimicrobial activities of Egyptian date palm (Phoenix dactylifera L.) fruits. Australian Journal of Basic and Applied Sciences. 2015;9(1):141-148.

24. Shahdadi F, Mirzaei HO, Daraei Garmakhany A. Study of phenolic compound and antioxidant activity of date fruit as a function of ripening stages and drying process. Journal of Food Science and Technology. 2015;52(3):1814-1819. https://doi.org/10.1007/s13197-013-1177-6

25. AlFaris NA, AlTamimi JZ, AlMousa LA, AlGhamidi FA, Alzaheb RA, Albarid NA. Antioxidant content determination in ripe date fruits (Phoenix dactylifera L.): A scoping review. Food Analytical Methods. 2021;14(5):897-921. https://doi.org/10.1007/s12161-020-01923-z

26. Hachani S, Hamia C, Boukhalkhal S, Silva AMS, Djeridane A, Yousfi M. Morphological, physico-chemical characteristics and effects of extraction solvents on UHPLC-DAD-ESI-MSn profiling of phenolic contents and antioxidant activities of five date cultivars (Phoenix dactylifera L.) growing in Algeria. NFS Journal. 2018;13:10-22. https://doi.org/10.1016/j.nfs.2018.10.001

27. Behravan M, Hossein Panahi A, Naghizadeh A, Ziaee M, Mahdavi R, Mirzapour A. Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. International Journal of Biological Macromolecules. 2019;124:148-154. https://doi.org/10.1016/j.ijbiomac.2018.11.101

28. Zhao M, Bai J, Bu X, Tang Y, Han W, Li D, et al. Microwave-assisted aqueous two-phase extraction of phenolic compounds from Ribes nigrum L. and its antibacterial effect on foodborne pathogens. Food Control. 2021;119. https://doi.org/10.1016/j.foodcont.2020.107449

29. Farhadi F, Khameneh B, Iranshahi M, Iranshahy M. Antibacterial activity of flavonoids and their structure-activity relationship: An update review. Phytotherapy Research. 2019;33(1):13-40. https://doi.org/10.1002/ptr.6208

30. Górniak I, Bartoszewski R, Króliczewski J. Comprehensive review of antimicrobial activities of plant flavonoids. Phytochemistry Reviews. 2019;18(1):241-272. https://doi.org/10.1007/s11101-018-9591-z

31. Abdullah N, Ishak NFM, Wan Shahida WS. In-vitro antibacterial activities of Ajwa date fruit (Phoenix dactylifera L.) extract against selected gram-negative bacteria causing gastroenteritis. International Journal of Pharmaceutical Sciences and Research. 2019;10(6):2951-2955. https://doi.org/10.13040/IJPSR.0975-8232.10(6).2951-55

32. Amin MU, Khurram M, Khan TA, Faidah HS, Shah ZU, Ur Rahman S, et al. Effects of luteolin and quercetin in combination with some conventional antibiotics against methicillin-resistant Staphylococcus aureus. International Journal of Molecular Sciences. 2016;17(11). https://doi.org/10.3390/ijms17111947

33. Tagousop CN, Tamokou J-D-D, Ekom SE, Ngnokam D, Voutquenne-Nazabadioko L. Antimicrobial activities of flavonoid glycosides from Graptophyllum grandulosum and their mechanism of antibacterial action. BMC Complementary Medicine and Therapies. 2018;18(1). https://doi.org/10.1186/s12906-018-2321-7

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Ali Haimoud S., Allem R. Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity // Foods and Raw Materials. 2023. no. 1. pp. 17-24. DOI: https://doi.org/10.21603/2308-4057-2023-1-549. (Date of access 06.09.2026).

MLA

Haimoud S. Ali, Allem R.. "Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity." Foods and Raw Materials 11.1 (2023): 17-24. DOI: https://doi.org/10.21603/2308-4057-2023-1-549.

APA

Ali Haimoud S., Allem R. (2023). Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity. Foods and Raw Materials, 11(1), 17-24. https://doi.org/10.21603/2308-4057-2023-1-549

Chicago

Haimoud S. Ali, Allem R.. "Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity." Foods and Raw Materials 11, no. 1 (2023): 17-24. https://doi.org/10.21603/2308-4057-2023-1-549.

WIKI

{{cite journal | author = Ali Haimoud S., Allem R. | title = Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity | journal = Foods and Raw Materials | year = 2023 | volume = 11 | issue = 1 | pages = 17-24 | doi = 10.21603/2308-4057-2023-1-549 }}

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ALGERIAN DATE PALM (PHOENIX DACTYLIFERA L.) FRUIT CULTIVARS: HPLC FINGERPRINTING AND ANTIBACTERIAL ACTIVITY Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity

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