Uglich, Russian Federation
Uglich, Russian Federation
Chlorine dioxide is a popular industrial disinfectant that possesses antimicrobial properties against various viruses and microorganisms, including gram-positive, gram-negative, and spore-forming bacteria. However, the available data become contradictory when it comes to the effect of pH on the bactericidal efficiency of chlorine dioxide against staphylococci and spore-forming microorganisms. The article describes the effect of pH 3–11 on the bactericidal efficiency of chlorine dioxide against gram-positive Staphylococcus aureus and spore-forming Bacillus subtilis. The initial contamination was 105 CFU/cm3. The effect of pH on the bactericidal properties of chlorine dioxide depended on the microorganism. The research revealed the complex effect of pH of the medium and the concentration of chlorine dioxide on the bactericidal efficiency against the gram-positive staphylococci and the sporeforming bacteria. For Staphylococcus aureus, a lower concentration of chlorine dioxide limited the effective range of active acidity with a shift to the acidic pH zone. The lowest concentration of chlorine dioxide enough to inhibit the growth of staphylococcus cells was 0.1 % at pH 3–5 while 0.5% proved effective throughout the entire pH range of 3–11. For Bacillus subtilis, the disinfecting effect did not depend on the pH of the medium when it remained within pH 3–9 and partially lost its efficiency at pH 10–11. At pH 3–9, the effective dosage of chlorine dioxide was ≥0.2% for Staphylococcus aureus and ≥0.5% for Bacillus subtilis.
chlorine dioxide, bactericidal efficiency, disinfection efficiency, test culture, Staphylococcus aureus , Bacillus subtilis, active acidity, disinfectant concentration
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