from 01.01.2009 until now
Kemerovo, Kemerovo, Russian Federation
Kemerovo, Kemerovo, Russian Federation
from 01.01.2022 until now
Kemerovo State Medical University
from 01.01.2008 to 01.01.2023
Kemerovo, Kemerovo, Russian Federation
Kemerovo, Kemerovo, Russian Federation
Kemerovo, Kemerovo, Russian Federation
Functional products containing natural antioxidants contribute to the improvement of human health indicators, and thus contribute to an increase in life expectancy. Milk is a valuable solubilizer that promotes the dissolution of insoluble substances in a liquid. The paper presents the results of a study under conditions of high-performance liquid chromatography (HPLC) with amperometric detection of antioxidant properties of secondary plant metabolites for the enrichment of dairy products. The objects of the study were biologically active compounds isolated from extracts of callus cultures of plants grown in vitro on liquid nutrient media. The use of HPLC chromatography with an amperometric glass-carbon detector to analyze the antioxidant properties of biologically active substances of plant origin made it possible to identify the following substances from 8 types of individual compounds with a pronounced potential for biological activity: rutin, rosemary acid from Pulmonaria officinalis, trans-cinnamic acid from Scutellaria baicalensis, rutin from Filipéndula ulmária. These compounds are able to effectively inhibit free radical processes in dairy products. The use of high-performance liquid chromatography with amperometric detection can be used as a criterion for evaluating the antioxidant properties of biologically active substances isolated from extracts of callus cultures.
dairy products; callus cultures; antioxidant activity; highly effective chromatography; biologically active substances
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